Part:BBa_K4774009:Design

CBD-Ssp-eGFP-Mxe-CBD2

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1926
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 588
  Illegal SpeI site found at 1926
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1926
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1926
  Illegal NgoMIV site found at 1430
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1510
  

Design Notes

The composite part combines Enhanced Green Fluorescent Protein (eGFP, BBa_K4401002), Ssp DnaB intein (BBa_K4774003), Mxe GyrA intein (BBa_K4774004), CBD (BBa_K4774002) and CBD2 (BBa_J70627)

Source

Genebank